Product description
Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. PHA-P is a mixture of isolectins containing L (leucoagglutinin) and E (erythroagglutinin) subunits (1) in compositions L1E3, L2E2, L3E1, E4.
Thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 128 kDa and specificity for complex oligosaccharides (1). PHA-P is not blood group specific (2) and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.
Medicago’s PHA-P lectin is supplied as a white to light-pink essentially salt-free lyophilized powder from 50 mM NH4HCO3, which evaporates during the lyophilization procedure. The purity of the lectin is determined by SDS-PAGE generating one single band. PHA-P is available in vials or plastic bottles containing 1 g, 10 mg or 5 mg of lyophilized powder and the product is to be used for laboratory work only.
Applications
- Lateral flow erythrocyte separation
Typical subunit composition
Total L subunit composition: 60-65%
Total E subunit composition: 35-40%
L4 25-35%
L3E1 20-30%
L2E2 10-20%
L1E3 17-27%
E4 1-11%
Directions for use
The lectin may be reconstituted in a suitable buffer for your application.
Shipping and storage
The product is shipped at -20°C, however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with PBS, pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months.
References
(1) Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)
(2) Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)
