Sodium Phosphate Buffer (NaPi)

Product description

Medicago’s Sodium Phosphate buffer is provided in different formulations in order to suit most biochemical and molecular biology applications. Choose a buffer with a pH and molarity that fits your laboratory procedures. The NaPi buffer is supplied as exactly pre-weighed powder in sealed pouches giving two volumes, 1000 ml and 5000 ml.

Dissolving the contents of one pouch in deionized water yields:

1. NaPi buffer 0.1 M with pH 6.0 at 25°C.
2. NaPi buffer 0.1 M or 1 M with pH 6.5 at 25°C.
3. NaPi buffer 0.1 M with pH 6.8 at 25°C.
4. NaPi buffer 0.1 M or 0.02 M with pH 7.0 at 25°C.
5. NaPi buffer 0.1 M or 1 M with pH 7.2 at 25°C.
6. NaPi buffer 0.1 M or 1 M with pH 7.4 at 25°C.
7. NaPi buffer 0.1 M with pH 7.5 at 25°C.
8. NaPi buffer 0.1 M with pH 8.0 at 25°C.

Applications:
Multi-purpose buffer for many laboratory procedures

Directions for use:
Empty one pouch of the NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200 ml to 300 ml of deionized water for a 1000 ml pouch and 1000 ml for a 5000 ml pouch and stir the solution for a few minutes. Adjust the volume up to 1000 ml or 5000 ml, stir and the buffer solution is ready to use.

Shipping and storage:
NaPi buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.

Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed powder mix
Volume: 1000 ml and 5000 ml

Concentration:

1. 0.1 M, pH 6.0 at 25°C
2. 0.1 M or 1 M, pH 6.5 at 25°C
3. 0.1 M, pH 6.8 at 25°C
4. 0.1 M or 0.02 M, pH 7.0 at 25°C
5. 0.1 M or 1 M, pH 7.2 at 25°C
6. 0.1 M or 1 M, pH 7.4 at 25°C
7. 0.1 M, pH 7.5 at 25°C
8. 0.1 M, pH 8.0 at 25°C

pH:

1. 6.0 ± 0.05 at 25°C
2. 6.5 ± 0.05 at 25°C
3. 6.8 ± 0.05 at 25°C
4. 7.0 ± 0.05 at 25°C
5. 7.2 ± 0.05 at 25°C
6. 7.4 ± 0.05 at 25°C
7. 7.5 ± 0.05 at 25°C
8. 8.0 ± 0.05 at 25°C