Product description
TBS Tween™20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phosphatase or peroxidase-conjugated antibodies.
Medicago’s TBS-T buffer is supplied in exactly pre-weighed tablets, each tablet giving 500 ml of 0.15 M NaCl, 0.05% TweenTM 20, 0.050 M Tris-HCl buffer solution with pH 7.6 at 25°C.
Applications
- Blocking buffer for plate based assays
- Wash buffer in immunoassays, for example ELISA and Western blotting
Directions for use
Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 200 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 500 ml, stir until full dissolution and the buffer is ready to use.
Shipping and storage
TBS-T buffer is shipped at room temperature. Store the tablets in a dry place at room temperature. Shelf life is two years after production date.
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition: 0.15 M NaCl, 0.050 M Tris-HCl, 0.05 % Tween™ 20
Volume: 500 ml
pH: 7.6 ± 0.1 at 25°C
