Product description
Tris-glycine-SDS buffer (TG-SDS) contains the denaturing agent sodium dodecyl sulphate (SDS). Protein electrophoresis under denaturing conditions (SDS-PAGE) involves separating proteins based on their size. By treating the sample under denaturing and reducing conditions with SDS, proteins unfold and become coated with SDS detergent molecules.
Medicago’s TG-SDS buffer is supplied as pre-weighed powder mixes in sealed pouches giving 1000 ml or 5000 ml of 0.025 M Tris, 0.192 M glycine, 0.10% SDS with pH 8.3 at 25°C.
Applications
- Protein electrophoresis
- Denatured protein electrophoresis
- Polyacrylamide gel electrophoresis
- Western blotting
Directions for use
Empty one pouch of TG-SDS buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes.
Adjust the volume up to 1000 ml or 5000 ml, stir until full dissolution and the buffer solution is ready to use.
Shipping and storage
The TG-SDS buffers are shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed powder mix
Concentration: 0.025 M Tris, 0.192 M glycine, 0.10% SDS
Volume: 1000 ml and 5000 ml
pH: 8.3 ± 0.2 at 25°C
