Product description
Ringer solution maintains the osmotic balance for bacteria. It is used
for the preparation of suspension of samples and other biological
matter in dilution techniques for enumeration. It is also used in the
microbial control of apparatus by the rinse and swab method.
Medicago’s Ringer solution is supplied as pre-weighed tablets in bottles.
One tablet yields 500 ml of buffer solution in the composition:
1 x solution; 2.25 g/L Sodium chloride, 0.105 g/L Potassium chlo
ride, 0.12 g/L Calcium chloride and, 0.05 g/L Sodium bicarbonate.
Applications
• Isotonic sample diluent
• Rinse solution for microbial control, the rinse and swab method
• Suspension of solid food and other specimens
• Diluent for pure bacteria colonies
Directions for use
Dissolve one tablet in 500 ml of deionized water. Stir until
full dissolution and sterilize by autoclaving at 121°C for 15 minutes if
needed.
Shipping and storage
Ringer solution is shipped at room temperature. Store the tablets in
a dry place at room temperature. Shelf life is three years.
Tips and hints
If the tablet is not properly dissolved, make sure:
– the water temperature is 22-25°C
– the buffer is properly stirred
– high quality deionized water is used
Sterilization can be performed by filtration or autoclaving. Filter the
buffer solution into a sterile flask through a 0.22 µm filter.
