Product description
Calmodulin is a bioactive protein isolated from bovine testes. The material is derived from individually marked animals born and raised in Sweden, a country where BSE has not been a problem. Calmodulin is a calcium-binding protein expressed in many eukaryotic cells. By binding to and regulating various protein targets, it affects cellular processes such as metabolism, nerve growth, apoptosis, inflammation, muscle contraction and memory.
Calmodulin is used by many proteins as a calcium sensor and signal transducer, as the proteins themselves are not able to bind calcium. The molecule can bind a maximum of four calcium ions and by undergoing post-translational modifications such as acetylation, phosphorylation, proteolytic cleavage and methylation, its functions can potentially be altered.
Calmodulin is supplied as a lyophilized white powder or flocculate from 50 mM NH4HCO3 with 10 μM CaCl2. No preservatives are added.
For laboratory use only.
Applications
- Protein calcium-binding studies on the regulation of a multitude of different protein targets
- Activation of cyclic nucleotide-dependent phosphodiesterase
- Co-factor/activator for kinase studies
- Studies of edema factor toxin and anthrax bacteria
Directions for use
Dissolve the protein in desired buffer depending on your application.
Shipping and storage
Stable for at least 5 years from production date when stored at -20°C. May be shipped at ambient temperature. After reconstitution in PBS pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Crystal structure of bovine Ca2+ calmodulin (1)
References
(1) A closed compact structure of native ca(2+)-calmodulin. Fallon, J.L., Quiocho, F.A.(2003) Structure 11: 1303 –7
